Application description
Uracil and Uridine are nucleosides which occur as a part of RNA. Both compounds are neutral in nature and very polar. Uracil is often used as a void marker in reversed-phase HPLC. A fast HILIC-based method was developed for analysis of uracil and uridine on the core-shell HILIC and cation-exchange column. Method provides good peak shape and retention control. Column and method can be used for analysis of polar compounds in HILIC, and HILIC/mixed-mode.
Conditions of Experiment
| Column: |
Coresep S |
| Separation Modes: |
HILIC |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
98% ACN with no additives |
| Detection: |
UV 250 nm |
| Sample: |
0.1-0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.5 mg/ml |
Analytes
| Class of compounds: |
Nucleobase, Nucleoside |
| Nature of compounds: |
Hydrophilic, Neutral |
| Compounds: |
Uracil, Uridine |
