Fast HPLC Analysis of Drug Composition on Coresep 100 Mixed-Mode Core-Shell Column

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: MeCN 15% with 0.1% TFA
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
Class of compounds: Amino acid, Aromatic compound, Drug, Supplement
Nature of compounds: Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Phenylalanine, Acetaminophen, Guaifenesin, Guaiacol