Fast HPLC Analysis of Drugs Codeine, Oxycodone, and Hydrocodone on Coresep 100 Mixed-Mode Core-Shell Column

Application description

Codeine, oxycodone and hydrocodone are opioids used to treat pain. These three drugs can be used in combination with other medications, such as acetaminophen and NSAIDs drugs. All three compounds are narcotic analgesics. Codeine is also used as an additive to drug formulations for suppression of cough. All three compounds are basic and hydrophobic in nature. They were separated on the Coresep 100 mixed-mode column through combination of strong reversed-phase and strong cation-exchange mechanisms. The retention time, resolution and peak shape are controlled by the amount of organic, buffer pH and buffer concentration. Various mobile phases can be used with the Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: MeCN 20% with 0.1% phosphoric acid
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic base, Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Codeine, Oxycodone, Hydrocodone