Fast HPLC Analysis of Isomers of Nitrophthalic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes

Application description

Ultra-fast method for separation of isomers of nitrophthalic acids was developed on a Coresep SB mixed-mode HPLC column. Compounds are separated based on weak reversed-phase and strong ion-exchange mechanisms. Nitrophthalic acids are used in synthesis of corrosion inhibitors, drugs, and agrochemicals. 3-Nitrophthalic and 4-nitrophthalic acids were separated within two minutes. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method can be used for analysis of various aromatic and aliphatic acids by reversed-phase anion-exchange mechanism, and can be applied to analysis of polar acids in various matrices. HPLC method is reproducible and robust.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 0.1% sulfuric acid
Detection: UV 250 nm, ELSD
Sample: 1 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Aromatic compound, Isomer
Nature of compounds: Acidic, Hydrophobic
Compounds: 3-Nitrophthalic acid, 4-Nitrophthalic acid