Application description
Ultra-fast method for separation of isomers of nitrophthalic acids was developed on a Coresep SB mixed-mode HPLC column. Compounds are separated based on weak reversed-phase and strong ion-exchange mechanisms. Nitrophthalic acids are used in synthesis of corrosion inhibitors, drugs, and agrochemicals. 3-Nitrophthalic and 4-nitrophthalic acids were separated within two minutes. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method can be used for analysis of various aromatic and aliphatic acids by reversed-phase anion-exchange mechanism, and can be applied to analysis of polar acids in various matrices. HPLC method is reproducible and robust.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
50% ACN with 0.1% sulfuric acid |
| Detection: |
UV 250 nm, ELSD |
| Sample: |
1 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic compound, Isomer, Organic acid |
| Nature of compounds: |
Acidic, Hydrophobic, Polar |
| Compounds: |
3-Nitrophthalic acid, 4-Nitrophthalic acid |
