HPLC Analysis of 2-Naphthoic Acid and Related Impurities on Coresep SB Mixed-Mode Column

Application description

2-Naphthoic acid and related impurities were analyzed on Coresep SB mixed-mode column. Method is fast, robust, reproducible, and can be used for analysis of 2-naphthoic acid and other organic acids in various sample matrices by mixed-mode reversed-phase anion-exchange chromatography. Various mobile phases can be used with Coresep SB column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Other compounds can be retained and separated on this mixed-mode column if they are hydrophobic and acidic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 40% ACN with 120 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Organic acid
Nature of compounds: Acidic, Hydrophobic
Compounds: 2-Naphthoic Acid