HPLC Analysis of 2,3-Dihydroxybenzoic Acid and Related Impurities on Coresep SB Mixed-Mode Column

Application description

2,3-dihydroxybenzoic acid is a natural phenolic acid. It is slightly hydrophobic (moderately hydrophilic) and acidic in nature. pKa of the acid is 2.56. 2,3-dihydroxybenzoic acid and related impurity was analyzed my mixed-mode chromatography using Coresep SB column. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is fast, robust and reproducible and can be used for analysis of isomers of other isomers of dihydroxybenzoic acid in various sample matrices. Various mobile phases can be used with Coresep SB column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and acidic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 40% ACN with 120 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Isomer, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,3-Dihydroxybenzoic acid