Application description
2,3-dihydroxybenzoic acid is a natural phenolic acid. It is slightly hydrophobic (moderately hydrophilic) and acidic in nature. pKa of the acid is 2.56. 2,3-dihydroxybenzoic acid and related impurity was analyzed my mixed-mode chromatography using Coresep SB column. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is fast, robust and reproducible and can be used for analysis of isomers of other isomers of dihydroxybenzoic acid in various sample matrices. Various mobile phases can be used with Coresep SB column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and acidic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
40% ACN with 120 mM AmFm pH 3 |
| Detection: |
UV 255 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Isomer, Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
2,3-Dihydroxybenzoic acid |
