HPLC Analysis of Acidic Drug Ketoprofen and Basic Counterions on Amaze SC Mixed-Mode Column

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN wiith 30 mM AmFm pH 3
Detection: UV 255 nm, ELSD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Potassium ion, Ketoprofen