HPLC Analysis of Antibacterial Drug Sulfamethazine and Related Impurities on Coresep 100 Mixed-Mode Column

HPLC Analysis of Antibacterial Drug Sulfamethazine and Related Impurities on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 10% ACN with 10 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Sulfamethazine
2. Related Impurities

Class of compounds: Antibiotic, Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

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