Application description
Sulfamethazine or sulfadimidine is a sulfonamide antibacterial drug. It is slightly hydrophobic and basic in nature. Sulfamethazine was analyzed on Coresep 100 HPLC column in reversed-phase and cation-exchange modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various basic drugs without the use of ion-pairing reagent. Mixed-mode columns have an ion-pairing reagent attached to the surface of silica gel which makes mixed-mode columns compatible with mass spectrometry and prep isolations.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.7 mL/min |
Analytes
| Class of compounds: |
Antibiotic, Aromatic base, Drug |
| Nature of compounds: |
Basic, Hydrophobic |
| Compounds: |
Sulfamethazine |
