HPLC Analysis of Antibacterial Drug Sulfamethazine on Coresep 100 Mixed-Mode Column

HPLC Analysis of Antibacterial Drug Sulfamethazine on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
1. Sulfamethazine

Class of compounds: Antibiotic, Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic

Application description

Sulfamethazine or sulfadimidine is a sulfonamide antibacterial drug. It is slightly hydrophobic and basic in nature. Sulfamethazine was analyzed on Coresep 100 HPLC column in reversed-phase and cation-exchange modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various basic drugs without the use of ion-pairing reagent. Mixed-mode columns have an ion-pairing reagent attached to the surface of silica gel which makes mixed-mode columns compatible with mass spectrometry and prep isolations.

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