HPLC Analysis of Antibiotic Cefalexin and Related Impurities on Coresep 100 Mixed-Mode Column
Cephalexin or Cefalexin is an antibiotic used to treat various infections. It kills gram-positive and some gram-negative bacteria by disrupting the growth of the bacterial cell wall. Cefalexin is a beta-lactam antibiotic within the class of first-generation cephalosporins. It works similarly to other agents within this class. Cephalexin is a hydrophobic zwitterionic compound and its impurities can be hydrophilic, hydrophobic, basic, acidic, and zwitterionic. Coresep 100 columns retain and separate these compounds without the use of ion-pairing reagents. Various buffers at different pH can be used to control the ionization state of the analytes and stationary phase. Coresep 100 columns can be used to analyze drugs and related impurities and provide perfect peak shape and retention control. The columns are suitable for robust method development and validation.
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange |
Column Dimenstions: | 4.6x150 mm, 2.7 um, 90A |
Mobile Phase: | A: 10% ACN with 10 mM NaH2PO4 pH 3.1 B: 40% ACN with 40 mM NaH2PO4 pH 3.1 from 100% A to 100% B in 8 min, 5 min hold |
Detection: | UV 275 |
Sample: | various |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Antibiotic, Aromatic acid, Aromatic base, Drug |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic |
Compounds: | Cefadroxil, Related Impurities |