HPLC Analysis of Antibiotic Chloramphenicol on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Antibiotic, Drug |
Nature of compounds: | Hydrophobic, Neutral |
Compounds: | Chloramphenicol |