HPLC Analysis of Antibiotic Chloramphenicol on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
1. Chloramphenicol

Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophobic, Neutral

Application description

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