HPLC Analysis of Antibiotic Nitrofurantoin on Heritage C18 Column According to US Pharmacopeia

Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm, 5 um, 100A
Mobile Phase: NaH2PO4 pH 7.0/MeCN 88/12
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Nitrofurantoin