HPLC Analysis of Antibiotic Phenoxymethylpenicillin on Heritage C18 Column

Conditions of Experiment
Column: Heritage C18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm, 5 um, 100A
Mobile Phase: 30% ACN with 30 mM NaH2PO4 pH 6.0
Detection: UV 270 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Antibiotic, Drug
Nature of compounds: Acidic, Hydrophobic
Compounds: Phenoxymethylpenicillin