HPLC Analysis of Antibiotic Sulfadiazine on Coresep 100 Mixed-Mode Column

HPLC Analysis of Antibiotic Sulfadiazine on Coresep 100 Mixed-Mode Column chromatogram
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Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: 10% ACN with 10 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
Class of compounds: Antibiotic, Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Sulfadiazine