HPLC Analysis of Antibiotic Sulfadiazine on Coresep 100 Mixed-Mode Column

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase and cation-exchange
Column Dimenstions:	3.2 x 100 mm
Mobile Phase:	10% ACN with 10 mM AmFm pH 3
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	0.6 mL/min

Analytes
1. Sulfadiazine
Class of compounds: Antibiotic, Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic

Application description