HPLC Analysis of Arginine and Related Impurities

HPLC Analysis of Arginine and Related Impurities chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: ACN gradient from 2% to 20%, H2SO4 from 0.02% to 0.2% in 12 minutes
Detection: UV 210 nm
Sample:
Injection:
Flow rate: 1.0 mL/min
Analytes
1. Glutamic acid
2. Citrulline
3. Ornithine
4. Lysine
5. Arginine

Class of compounds: Amino acid, Aromatic acid, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic

Application description

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