HPLC Analysis of Arginine and Related Impurities

Conditions of Experiment
Column: Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: ACN gradient from 2% to 20%, H2SO4 from 0.02% to 0.2% in 12 minutes
Detection: UV 210 nm
Sample:
Injection:
Flow rate: 1.0 mL/min
Analytes
Class of compounds: Amino acid, Aromatic acid, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Glutamic acid, Citrulline, Ornithine, Lysine, Arginine