HPLC Analysis of Ascorbic and Citric Acids in HILIC/Anion-Exclusion Modes on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: 90% ACN, no additives
Detection: 210 nm
Sample: 1 ml/min
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Organic acid, Preservative, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Citric acid, Ascorbic acid