Home HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column
Conditions of Experiment
| Column: |
Heritage MA |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
4.6x50 mm, 3 μm, 100A |
| Mobile Phase: |
ACN gradient from 2% to 40%, phosphoric acid gradient from 0.03% to 0.4% in 5 min, 2 min hold |
| Detection: |
UV 220 nm |
| Sample: |
1 mg/ml |
| Injection: |
2 μL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Organic acid, Preservative, Supplement, Vitamin |
| Nature of compounds: |
Acidic, Hydrophilic, Polar, Zwitterionic |
| Compounds: |
Ascorbic acid, Citric acid, Ethylenediaminetetraacetic acid |
