HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column

HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column chromatogram

Conditions of Experiment

Column:	Heritage N
Separation Modes:	HILIC, anion-exclusion
Column Dimenstions:	4.6 x 150 mm, 5 um, 100A
Mobile Phase:	75% ACN with 15 mM AmAc pH 7
Detection:	ELSD
Sample:	0.3 mg/ml
Injection:	5 uL
Flow rate:	1 ml/min

Analytes
1. Ascorbic acid 2. Methylmalonic acid 3. Succinic acid
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar

Application description

Heritage N purchase