HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column

HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column chromatogram
Conditions of Experiment
Column:
Heritage N
Separation Modes:
HILIC, anion-exclusion
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 75% ACN with 15 mM AmAc pH 7
Detection: ELSD
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
1. Ascorbic acid
2. Methylmalonic acid
3. Succinic acid

Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar

Application description

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