HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column

Application description

Ascorbic, succinic and malonic acid are weak organic acids. Ascorbic acid is a vitamin found in various foods. It is one of the more important supplements used in various formulations. Food containing Vitamin C includes fruits and vegetables. It is a very important nutrient for humans and animals. Vitamin C functions as a cofactor in many enzymatic reactions in humans and animals. It mediates a variety of essential biological functions like wound healing and collagen synthesis. Methylmalonic acid is a dicarboxylic acid. Succinic acid is a dicarboxylic acid which is used in the production of some polymers and component of some alkyl resins. It is also used as a food additive and dietary supplement. It serves as an acidity regulator. We have developed an HILIC anion-exclusion approach for the analysis of these three organic acids on Heritage N HPLC column. The retention time is controlled by the amount of ACN and the amount of buffer. No ion-pairing reagent is required. Various buffers can be used depending on your detection technique

Conditions of Experiment
Column: Heritage N
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 75% ACN with 15 mM AmAc pH 7
Detection: ELSD
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Ascorbic acid, Methylmalonic acid, Succinic acid