HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column

Conditions of Experiment
Column: Heritage N
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 75% ACN with 15 mM AmAc pH 7
Detection: ELSD
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Ascorbic acid, Methylmalonic acid, Succinic acid