HPLC Analysis of Atenolol and Related Impurities on Core-Shell Mixed-Mode Coresep 100 Column

Application description

Atenolol and related impurities were analyzed on the Coresep 100 core-shell mixed-mode reversed-phase cation-exchange column. Atenolol is a beta blocker which is used to treat cardiovascular diseases. Atenolol is used to treat angina, hypertension, long QT syndrome and is also used to treat symptoms of alcohol withdrawal. Atenolol is a hydrophobic basic compound containing aromatic ring with lipophilic and hydrophilic substitute groups, along with a secondary amine in the side chain. It is retained by a combination of reversed-phase and cation exchange mechanisms. The retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. The method is fast, robust and reproducible, so it can be used for analysis of atenolol in various matrices. The method is compatible with all detection techniques (UV, ELSD, CAD, MS, RI). These core-shell mixed-mode columns provide a great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: MeCN 40% with 30 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Atenolol