HPLC Analysis of Azithromycin on Amaze HD Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: ACN/water/buffer
Detection: ELSD, 45*C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Antibiotic, Drug
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Azithromycin