Application description
Cefadroxil is a broad-spectrum antibiotic effective in the treatment of Gram-positive and Gram-negative infections. It is an organic zwitterionic compound with medium hydrophobicity. We have developed a method for analysis of this antibiotic on Coresep 100 core-shell reversed-phase cation-exchange column. It retains compounds based on RP and cation-exchange mechanisms. Retention of the antibiotic and related impurities can be effectively controlled by the amount of ACN, buffer concentration, and buffer pH. The choice of the buffer depends on the sample matrix and detection technique. Column and method are compatible with common detection techniques like UV, ELSD, CAD, MS, and RI. The method is robust and reproducible and can be used to analyze complex mixtures either in a single or dual mode. Mixed-mode HPLC columns from HElIX offer robust and reliable separation. Peak shapes are symmetrical and demonstrate high efficiency attributed to core-shell particles. Mixed-mode HPLC columns are a good choice for method development and validation for various drugs, drug composition, and remedies.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase, cation-exchange |
| Column Dimenstions: |
4.6x150 mm, 2.7 um, 90A |
| Mobile Phase: |
A: 10% ACN with 10 mM NaH2PO4 pH 3.1
B: 40% ACN with 40 mM NaH2PO4 pH 3.1
from 100% A to 100% B in 8 min, 5 min hold |
| Detection: |
UV 255 |
| Sample: |
2 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Antibiotic, Aromatic acid, Aromatic base, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic |
| Compounds: |
Cefadroxil, Related Impurities |
