HPLC Analysis of Broad Spectrum Antibiotic Cefadroxil and Related Impurities on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 10 mM NaH2PO4 pH 3.1
B: 40% ACN with 40 mM NaH2PO4 pH 3.1
from 100% A to 100% B in 8 min, 5 min hold
Detection: UV 255
Sample: 2 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Antibiotic, Drug
Nature of compounds: Basic, Hydrophilic
Compounds: Cefadroxil