HPLC Analysis of Bromate and Chlorate Ions on Heritage MA Mixed-Mode Column

Application description

Chlorate and bromate are polar inorganic acidic ions. We developed a separation approach on the Heritage MA column for the separation of these two anions. Both compounds are retained by a pure ion-exchange mechanism. The retention time can be adjusted by the buffer pH and buffer concentrations. These and other ions can be analyzed in one run in an isocratic or gradient mode. This method is compatible with MS, ELSD, CAD and RI detection techniques. This method can be used for the analysis of inorganic and inorganic anions in various matrices. Basic drugs and counterions can be separated in one run on this column. The basic drug needs to be hydrophobic enough to compensate for the presence of a cation-exclusion mechanism on the Heritage MA column.

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 60 mM AmFm pH 3
Detection: ELSD, 50°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic anion, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Bromate Ion, Chlorate ion