HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia

Conditions of Experiment

Column:	Heritage С18
Separation Modes:	reversed-phase
Column Dimenstions:	4.6 x 150 mm, 5 um, 100A
Mobile Phase:	NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid
Detection:	UV 270 nm
Sample:	0.2 mg/ml
Injection:	5 uL
Flow rate:	1 mL/min

Analytes