HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia

Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid
Detection: UV 270 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Stimulant, Xanthine
Nature of compounds: Hydrophilic, Neutral
Compounds: Caffeine