HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 150 mm, 5 um, 100A |
Mobile Phase: | NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid |
Detection: | UV 270 nm |
Sample: | 0.2 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Stimulant, Xanthine |
Nature of compounds: | Hydrophilic, Neutral |
Compounds: | Caffeine |