HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia

HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia chromatogram
Conditions of Experiment
Column:
Heritage С18
Separation Modes:
reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid
Detection: UV 270 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
1. Caffeine

Class of compounds: Stimulant, Xanthine
Nature of compounds: Hydrophilic, Neutral

Application description

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