HPLC Analysis of Calcium Gluconate Formulation on Amaze HD Mixed-Mode Columns

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6x150 mm, 3 um , 100A
Mobile Phase: 90% ACN witj 0.05% phosphoric acid
Detection: 205 nm
Sample: 0.3-6 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid, Sugar
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Gluconic acid, Saccharic acid