HPLC Analysis of Camphor-10-Sulfonic Acid on Amaze TH Mixed-Mode Column

Application description

Camphor-10 Sulfonic acid is a strong organosulfur organic acid. Camphorsulfonic acid is used in pharmaceutical formulations like trimethaphan camsilate and lanabecestat camsylate. The compound is slightly hydrophobic and acidic. We have developed an anion-exchange method using the Amaze TH column. The Amaze TH mixed-mode column retains compounds in HILIC, cation- and anion-exchange modes. At lower concentrations of acetonitrile, it behaves like a cation- and anion-exchange column. The retention time in anion- and cation-exchange modes is adjusted by the amount of buffer and buffer pH. The amount of ACN and buffer properties affect the ionization state of the stationary phase and analyte. The method and column are suitable for the analysis of polar neutral, polar acidic, polar basic, hydrophobic acidic and hydrophobic basic compounds. This approach is compatible with all major detection techniques (UV, ELSD, CAD, RI and MS detection)

Conditions of Experiment
Column: Amaze TH
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 40% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid
Nature of compounds: Acidic, Hydrophilic
Compounds: Camphor-10-sulfonic Acid