HPLC Analysis of Cefepime and Related Impurities on Coresep 100 Mixed-Mode Core-Shell Column

Application description

Cefepime is a cephalosporin antibiotic. It is used to treat moderate to severe pneumonia and various infections. Cefepime is a polar zwitter-ionic compound which is zwitterionic. It contains a carboxylic acid fragment as well as a quaternary amine. We have developed a mixed-mode reversed--phase cation exchange approach using our Coresep 100 HPLC column. Cefepime is retained by the combination of reversed-phased and cation-exchange mechanisms. No ion-pairing reagent is required to retain this polar ionic drug. The retention time of cefepime and related impurities can be adjusted by the amount of ACN, amount of buffer and buffer pH. The pH of the mobile phase will affect the ionization state of the analyte and ionization state of stationary phase. This method is robust, reproducible and mass-spectrometry compatible. The Coresep 100 mixed-mode column can be used in various modes of separation for analysis of polar, non-polar, ionizable and non-ionizable compounds

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 0.05% H2SO4, B: 50% ACN with 0.2% H2SO4, from 100% A to 100% B in 12 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Cefepime (antibiotic)