HPLC Analysis of Cefepime and Related Impurities on Coresep 100 Mixed-Mode Core-Shell Column

Conditions of Experiment
Column: Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 0.05% H2SO4, B: 50% ACN with 0.2% H2SO4, from 100% A to 100% B in 12 min with 3 min hold
Detection: UV 275 nm
Flow rate:
Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Cefepime (antibiotic)