Application description
Cetylpyridinium chloride is a surfactant widely used in various industries. It is hydrophobic and basic in nature. Cetylpyridinium demonstrates poor performance and peak shape in reversed-phase chromatography due to residual silanol interactions. In mixed-mode chromatography a polar ionizable group on the surface can prevent interaction between basic molecule like cetylpyridinium and residual silanols. Absence of silanol interactions guarantees a better than in RP chromatography peak shape. Mixed-mode HILIC approach allows to retain compounds either based on multiple or single mechanisms interaction, thus providing a valuable approach for analysis. The method can be used for analysis of other hydrophobic and basic surfactants.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
RP, cation-exclusion |
| Column Dimenstions: |
3.0 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
ACN/water/phosphoric acid |
| Detection: |
UV 255 nm |
| Sample: |
0.2 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Aromatic base, Surfactant |
| Nature of compounds: |
Basic, Hydrophobic |
| Compounds: |
Cetylpyridinium |
