HPLC Analysis of Cheratussin AC Cough Syrup on Coresep 100 Mixed-Mode Column

Application description

Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. The active ingredients are guaifenesin and codeine. Codeine is a narcotic cough suppressant. It affects the signals in the brain that trigger cough reflex. Guaifenesin is an expectorant. It helps loosen congestion in your chest and throat, making it easier to cough out through your mouth. Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. Codeine is a highly hydrophobic and basic compound and guaifenesin is a neutral hydrophilic compound. The highly effective separation was achieved on a Coresep 100 mixed-mode HPLC column. The column is compatible with 100% ACN and 100% water due to the presence of hydrophilic ionizable groups on the surface of core-shell particles. Coresep 100 resist phase collapse which is typical for pure reversed-phase columns. Method and Coresep 100 column can be used for the analysis of various drugs and drug composition where alternative selectivity is required to achieve baseline separation. Method and column can be easily validated. Results are reproducible and robust