HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Gradient Mode

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/AmAc gradient
Detection: ELSD, 40*C
Sample: 2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine