HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Isocratic Mode

Conditions of Experiment
Column: Anaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 4
Detection: ELSD, 40*C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine