HPLC Analysis of Dextromethorphan and Related Impurities on Amaze SC Mixed-Mode Column
Conditions of Experiment
| Column: | Amaze SC |
| Separation Modes: | reversed-phase, cation-exchange |
| Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
| Mobile Phase: | ACN/Water/Ammonium phosphate gradient |
| Detection: | 280 nm |
| Sample: | 0.1-0.3 mg/ml |
| Injection: | 5 uL |
| Flow rate: | 0.6 ml/min |
Analytes
| Class of compounds: | Amines, Aromatic base, Drug |
| Nature of compounds: | Basic, Hydrophilic, Hydrophobic |
| Compounds: | Ent-3-Methoxymorphinan, ent-(14S)-3-Methoxy-17-Methylmorphinian, 10-Keto dextromethorphan, Dextromethorphan |