HPLC Analysis of Dextromethorphan and Related Impurities on Amaze SC Mixed-Mode Column

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/Water/Ammonium phosphate gradient
Detection: 280 nm
Sample: 0.1-0.3 mg/ml
Injection: 5 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Ent-3-Methoxymorphinan, ent-(14S)-3-Methoxy-17-Methylmorphinian, 10-Keto dextromethorphan, Dextromethorphan