HPLC Analysis of Divalent Cations and Corresponding Counterions on Amaze TCH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, anion-exchange, cations-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN
from 75% to 30%, AmFm from 40 mM pH 4.0 to 40 mM pH 2.5
Detection: ELSD
Sample: 0.3-0.5 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Contaminant, Inorganic acid, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Nitrate ion, Bromide ion, Bromate Ion, Calcium ion, Persulfate ion, Magnesium ion, Phosphate Ion, Nickel ion, Copper ion, Zinc ion, Barium ion