HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A