HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 72% ACN with 56 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine