HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange and anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 72% ACN with 56 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |