HPLC Analysis of Dopamine and 5 Amino Acids in Reversed-Phase and Cation-Exchange Modes on Amaze SC Column
Conditions of Experiment
| Column: | Amaze SC |
| Separation Modes: | reversed-phase and cation-exchange |
| Column Dimenstions: | 3.0x100 mm, 5μm, 100A |
| Mobile Phase: | 10% ACN with 20 mM AmFm pH 3 |
| Detection: | Corona CAD |
| Sample: | 0.5 mg/ml |
| Injection: | 2 μL |
| Flow rate: | 0.6 ml/min |
Analytes
| Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
| Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |