HPLC Analysis of Drug Amphetamine and Related Impurities on Coresep 100 Mixed-Mode Column

Application description

Amphetamine is a stimulant of the phenethylamine class found in ADHD-treatment drugs such as Adderall®, Vyvanse®, and ProCentra®. Because of its stimulant effect, amphetamine is used recreationally as a performance-enhancer and an illicit drug. It is a potent stimulant and a Schedule II drug. Amphetamine properly refers to a specific chemical, the racemic free base, which is equal parts of the two enantiomers, levoamphetamine and dextroamphetamine, in their pure amine forms. It is slightly hydrophobic and basic in nature. Amphetamine and related impurities were analyzed without the use of ion-pairing reagent on Coresep 100 mixed-mode HPLC column. Amphetamine is retained by weak reversed-phase mechanism and by strong cation-exchange mechanism. Method is fast, robust, reproducible, and can be used for analysis of amphetamine and other hydrophilic basic drugs by mixed-mode chromatography in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 30% ACN with 0.1% TFA
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.5 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug, Stimulant
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Amphetamine, Related Impurities