HPLC Analysis of Drug Apixaban and Related Impurities on Heritage MA Mixed-Mode Columns

Conditions of Experiment
Column: Heritage MA
Separation Modes: reverse-phase, cation-exclusion
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: "ACN gradient
from 15% to 60% in 20 min, 0.1% H3PO4 "
Detection: 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Apixaban, Related Impurities