HPLC Analysis of Drug Bupropion and Related Impurities on Coresep 100 Mixed-Mode Column

Application description

Bupropion is a medication primarily used as an antidepressant and smoking cessation aid. It is an effective antidepressant on its own, but is also used as an add-on medication in cases of incomplete response to first-line SSRI antidepressants.Common side effects include dry mouth, trouble sleeping, agitation, and headaches. It is a norepinephrine-dopamine reuptake inhibitor (NDRI) and a nicotinic receptor antagonist. It is an atypical antidepressant, different from most others. Bupropion is classified as cathinones and is similar in structure to substituted phenethylamines. Bupropion and related impurities were separated by mixed-mode chromatography without the use of ion-pairing reagents (IP reagent). Compounds are retained on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer, and buffer pH. Various mobile phases can be used with Coresep 100 column to accommodate various detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 30% ACN with 0.1% TFA
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.5 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Bupropion, Related Impurities