HPLC Analysis of Drug Cefuroxime and Related Impurities on Coresep 100 Mixed-Mode Column

Application description

Cefuroxime is the second generation cephalosporin antibiotic. It is hydrophobic and acidic in nature. Cefuroxime and related impurities were analyzed by mixed-mode HPLC on Coresep 100 column. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. The method is fast, robust, reproducible, and can be used for analysis of cefuroxime in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 35% ACN with 0.1% H3PO4
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Antibiotic, Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cefuroxime (two isomers), Related Impurities