Application description
Cefuroxime is the second generation cephalosporin antibiotic. It is hydrophobic and acidic in nature. Cefuroxime and related impurities were analyzed by mixed-mode HPLC on Coresep 100 column. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. The method is fast, robust, reproducible, and can be used for analysis of cefuroxime in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and anion-exclusion |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
35% ACN with 0.1% H3PO4 |
| Detection: |
UV 255 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 mL/min |
Analytes
| Class of compounds: |
Antibiotic, Aromatic acid, Aromatic base, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic |
| Compounds: |
Cefuroxime (two isomers), Related Impurities |
