HPLC Analysis of Drug Celebrex and Related Impurities on Core-Shell Mixed-Mode Coresep 100 Column

Application description

The drug Celebrex and related impurities were analyzed on the core-shell mixed-mode reversed-phase cation-exchange Coresep 100 column. Celebrex Celecoxib is COX-2 selective NSAIDS, which is used for the treatment of arthritis and acute pain. Celebrex is a hydrophobic neutral compound which is retained on the Coresep 100 column by a reversed-phase mechanism. Ionizable impurities are retained and separated by reversed-phase and cation-exchange mechanism (basic impurities), along with reversed-phase anion-exclusion mechanisms. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, so it can be used for analysis of the drug Celebrex in various matrices (reaction mixtures, formulations, biofluids). The method is compatible with all detection techniques (UV, ELSD, CAD, MS, RI). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: MeCN 40% with 30 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: Celebrex