HPLC Analysis of Drug Cetirizine and Related Impurities on Coresep SB Mixed-Mode Column

Application description

Cetirizine is an antihistamine drug used for the treatment of fevers, allergies, angioedema, and urticaria. It acts as a selective antagonist of the histamine H1 receptor. It is a non-sedating, histamine H1-receptor antagonist and major metabolite of Hydroxyzine. It is a hydrophobic and zwitterionic compound. The HPLC analysis of Cetirizine on the Coresep SB column is robust and reproducible with a perfect peak shape for the main analyte. The residual silanols are shielded by a positively charged group on the surface of silica gel. Cetirizine and related impurities were separated based on hydrophobic and ionic properties of API and impurities. This method can be used for the analysis of hydrophobic basic compounds in reversed-phase cation-exclusion mode. Various mobile phases can be used with Coresep SB column to accommodate differing detection techniques (UV, MS, CAD, ELSD detection). The Coresep SB is a perfect choice for the analysis of hydrophobic basic compounds as well as neutral and polar acidic compounds. The retention time is controlled by a mobile phase composition. The amount of ACN, buffer pH and buffer concentration will affect the elution and separation between compounds. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 15% ACN with 0.2% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Cetirizine, Related Impurities