HPLC Analysis of Drug Dexamethasone and Related Impurities on Coresep 100 Mixed-Mode Column

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase and cation-exchange
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes
1. Dexamethasone 2. Related Impurities
Class of compounds: Aromatic acid, Aromatic base, Drug, Steroids
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral

Application description