Home HPLC Analysis of Drug Dexamethasone and Related Impurities on Coresep 100 Mixed-Mode Column
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.7 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic base, Drug, Steroids |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Neutral |
| Compounds: |
Dexamethasone, Related Impurities |
