HPLC Analysis of Drug Dextromethorphan on Heritage MA Column. Effect of Acetonitrile and Buffer Concentration on Retention Time

Application description

Dextromethorphan is a drug of the morphinan class with sedative, dissociative, and stimulant properties (at higher doses). It is a cough suppressant in many over-the-counter (OTC) cold and cough medicines including Benylin DM, Mucinex DM, Robitussin, NyQuil, Dimetapp, Vicks, Coricidin, Delsym, TheraFlu, Cheracol D, and others. Dextromethorphan has also found numerous other uses in medicine, ranging from pain relief (as either the primary analgesic or an opioid potentiator) over psychological applications to the treatment of addiction. Dextromethorphan and related impurities were analyzed by mixed-mode chromatography on Heritage MA HPLC column. Compounds are separated by reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 15% ACN with 30 mM AmAc pH 4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Amines, Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Dextromethorphan