HPLC Analysis of Drug Diazepam and Related Impurities on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 40% ACN with 0.1% H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral
Compounds: Diazepam, Related Impurities