HPLC Analysis of Drug Escitalopram on Coresep SB Mixed-Mode Column

Application description

Escitalopram is an antidepressant medication mainly used for major depressive disorders. Escilatopram is a selective Serotonin reuptake inhibitor. The compound is hydrophobic and basic in nature. The HPLC method was developed for the HPLC analysis of Escitalopram on Coresep SB mixed-mode column. The method is robust with a good peak shape for the main compound. There is no interaction between residual silanols on the surface of the stationary phase and this basic hydrophobic compound. The residual silanols are shielded by a positively charged group on the surface of silica gel. This method can be used for the analysis of hydrophobic basic compounds in reversed-phase cation-exclusion mode. Various mobile phases can be used with the Coresep SB column to accommodate multiple detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 5% ACN with 0.2% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Escitalopram