HPLC Analysis of Drug Hydrocodone on Coresep 100 Mixed-Mode Column

Application description

Hydrocodone is a semisynthetic, moderately potent opioid which is often used with acetaminophen for treatment of acute and chronic pain. It is sold under the brands Vicodin, Norco, Lortab, Zohydro and others. Hydrocodone was analyzed on Coresep 100 multi-mode HPLC column. The compound is hydrophobic and basic in nature and is retained by a combination of strong reversed-phase and strong cation-exchange mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. This robust method can be used for quantitative analysis of hydrocodone in biofluids and formulation using any detection technique including mass spectrometry. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 60% ACN with 30 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Class of compounds: Aromatic base, Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Hydrocodone