HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column According to US Pharmacopeia

HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column According to US Pharmacopeia chromatogram
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Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 100 mm, 5 um, 100A
Mobile Phase: MeCN/H2O 25/75
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Drug, Steroids
Nature of compounds: Hydrophobic, Neutral
Compounds: Hydrocortisone