HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column

HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column chromatogram
Conditions of Experiment
Column:
Heiratge C18
Separation Modes:
reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 40% ACN with 10 mM NaH2PO4 pH 2.5
Detection: UV 210 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
1. Hydrocortisone

Class of compounds: Drug, Steroids
Nature of compounds: Hydrophobic, Neutral

Application description

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